Trichome

Authors
ALAN P POLAND, DANIEL W NEBERT
Publication date
1973/1/1
Journal
Journal of Pharmacology and Experimental Therapeutics
Volume
184
Issue
1
Pages
269-277
Publisher
American Society for Pharmacology and Experimental Therapeutics
Description
POLAND, ALAN P. AND DANIEL W. NEBERT: A sensitive radiameetric assay of aminopyrine N-demethylation. J. Pharmacol. Exp. Them. 184: 269-277, 1973.
A simple radiometric assay for determining aminopyrine N-demethylase activity is described. The principle of partitioning the polar“C-formaldehyde metabolite complexed with semicarbazide from the lipophilic nonmetabolized substrate(methyl-4C-labeled aminopyrine) was utilized. This radioisotopic assay correlates well with the standard colorimetric method and is at least 100 times as sensitive. Kinetic evidence for two aminopyrine N-demethylase activities is shown. With the use of this radiometric assay, a stimulation of aminopyrine N-demethylatian in fetal chick hepatic and nonhepatic tissues by prior treatment with phenobarbital, allylisopropylacetamide or 3-methylcholanthrene is detectable.
Total citations
1984198519861987198819891990199119921993199419951996199719981999200020012002200320042005200620072008200920102011201220132014201520162017201820192020202120222023757351113212111111111
Scholar articles
AP POLAND, DW NEBERT - Journal of Pharmacology and Experimental …, 1973

Leave a Reply