Knowsley can refer to:

England

• Knowsley, Merseyside, village
• Metropolitan Borough of Knowsley, local government district
• Knowsley Safari Park
• Knowsley (UK Parliament constituency), current parliamentary constituency
• Knowsley North and Sefton East (UK Parliament constituency), former parliamentary constituency (1997–2010)
• Knowsley North (UK Parliament constituency), former parliamentary constituency (1983–1997)
• Knowsley South (UK Parliament constituency), former parliamentary constituency (1983–2010)
• Knowsley Hall, stately home
• Knowsley Community College, further education college
• Knowsley Road, former home stadium of St Helens RFC (rugby league)

Australia

• Knowsley, Victoria, hamlet