Holston may refer to:

• Holston, Virginia
• Holston River
• Holston Formation
• Treaty of Holston
• Holston Conference, an annual conference within Methodism
• Holston Army Ammunition Plant in Kingsport, Tennessee
• The Holston, an NRHP-listed high-rise in Knoxville, Tennessee

People with the surname[edit]

• David Holston (born 1986), American basketball player
• Mike Holston (born 1958), American football player