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{{Expert-subject|Genetics|date=February 2009}} |
{{Expert-subject|Genetics|date=February 2009}} |
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[[Image:Green4.jpg|thumb|right|300px|Front: Scanning electron micrograph of hamster ovary cells (CHO) following impalement on a nanofiber array. Background: Optical microscope image of a transformed colony of CHO expressing green fluorescent protein from nanofiber delivered plasmids 22 days following impalement upon DNA modified nanofiber array. |
[[Image:Green4.jpg|thumb|right|300px|Front: Scanning electron micrograph of hamster ovary cells (CHO) following impalement on a nanofiber array. Background: Optical microscope image of a transformed colony of CHO expressing green fluorescent protein from nanofiber delivered plasmids 22 days following impalement upon DNA modified nanofiber array. |
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]]'''Impalefection''' is a method of [[gene delivery]] using [[nanomaterial]]s, such as [[carbon nanofiber]]s, [[nanotube]]s, [[nanowire]]s Ref.1. Needle-like nanostructures are synthesized perpendicular to the surface of a substrate. Plasmid DNA containing the gene, intended for intracellular delivery, is attached to the nanostructure surface. A chip with arrays of these needles is then pressed against cells or tissue. Cells that are impaled by nanostructures can express the delivered gene(s). |
]]'''Impalefection''' is a method of [[gene delivery]] using [[nanomaterial]]s, such as [[carbon nanofiber]]s, [[carbon nanotube]]s, [[nanowire]]s Ref.1. Needle-like nanostructures are synthesized perpendicular to the surface of a substrate. Plasmid DNA containing the gene, intended for intracellular delivery, is attached to the nanostructure surface. A chip with arrays of these needles is then pressed against cells or tissue. Cells that are impaled by nanostructures can express the delivered gene(s). |
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As one of the types of [[transfection]], the term is derived from two words - [[impalement]] and [[infection]]. |
As one of the types of [[transfection]], the term is derived from two words - [[impalement]] and [[infection]]. |
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Revision as of 08:57, 16 June 2010
Impalefection is a method of gene delivery using nanomaterials, such as carbon nanofibers, carbon nanotubes, nanowires Ref.1. Needle-like nanostructures are synthesized perpendicular to the surface of a substrate. Plasmid DNA containing the gene, intended for intracellular delivery, is attached to the nanostructure surface. A chip with arrays of these needles is then pressed against cells or tissue. Cells that are impaled by nanostructures can express the delivered gene(s).
As one of the types of transfection, the term is derived from two words - impalement and infection.
Applications
One of the features of impalefection is spatially resolved gene delivery that holds potential for such tissue engineering approaches in wound healing as gene activated matrix technology Ref2.
Carrier materials
Vertically aligned carbon nanofiber arrays prepared by photolithography and plasma enhanced chemcial vapor deposition are one of the suitable types of material. Silicon nanowires is another choice of nanoneedles that have been utilized for impalefection (Ref)
References
1. Tracking gene expression after DNA delivery using spatially indexed nanofiber arrays McKnight, T.E., A.V. Melechko, D.K. Hensley, D.G.J. Mann, G.D. Griffin, and M.L. Simpson, . Nano Letters, 2004. 4(7): p. 1213-1219
2. Bonadio J. (2000) Local gene delivery for tissue regeneation. e-biomed. J Regener Med 1:25-29
See also
External links
- "An Attempted Nanowire Impalefection of Nerve Cells"
- "Intracellular integration of synthetic nanostructures with viable cells for controlled biochemical manipulation"