Spiral architect E5I5O (talk | contribs) m Fixed typos and altered the text grammatically. Also added in the qualifier that cells that are impaled 'CAN' express the delivered genes,. |
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'''Impalefection''' is a method of [[gene delivery]] using [[nanomaterial]]s, such as [[carbon nanofiber]]s, [[nanotube]]s, [[nanowire]]s Ref.1. Needle-like nanostructures are synthesized perpendicular to the surface of a substrate. Plasmid DNA containing the gene, intended for |
'''Impalefection''' is a method of [[gene delivery]] using [[nanomaterial]]s, such as [[carbon nanofiber]]s, [[nanotube]]s, [[nanowire]]s Ref.1. Needle-like nanostructures are synthesized perpendicular to the surface of a substrate. Plasmid DNA containing the gene, intended for intracellular delivery, is attached to the nanostructure surface. A chip with arrays of these needles is then pressed against cells or tissue. Cells that are impaled by nanostructures can express the delivered gene(s). |
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==Etymology== |
==Etymology== |
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Revision as of 17:28, 20 March 2007
Impalefection is a method of gene delivery using nanomaterials, such as carbon nanofibers, nanotubes, nanowires Ref.1. Needle-like nanostructures are synthesized perpendicular to the surface of a substrate. Plasmid DNA containing the gene, intended for intracellular delivery, is attached to the nanostructure surface. A chip with arrays of these needles is then pressed against cells or tissue. Cells that are impaled by nanostructures can express the delivered gene(s).
Etymology
Similar to transfection, the term is derived from two words - impalement and infection.
References
1. Tracking gene expression after DNA delivery using spatially indexed nanofiber arrays McKnight, T.E., A.V. Melechko, D.K. Hensley, D.G.J. Mann, G.D. Griffin, and M.L. Simpson, . Nano Letters, 2004. 4(7): p. 1213-1219
External links
- "An Attempted Nanowire Impalefection of Nerve Cells"
- "Intracellular integration of synthetic nanostructures with viable cells for controlled biochemical manipulation"