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| ⚫ | <!-- Deleted image removed: [[Image:Green4.jpg|thumb|right|300px|Front: Scanning electron micrograph of hamster ovary cells (CHO) following impalement on a nanofiber array. Background: Optical microscope image of a transformed colony of CHO expressing green fluorescent protein from nanofiber delivered plasmids 22 days following impalement upon DNA modified nanofiber array.]] --> |
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| ⚫ | '''Impalefection''' is a method of [[gene delivery]] using [[nanomaterial]]s, such as [[carbon nanofiber]]s, [[carbon nanotube]]s, [[nanowire]]s.<ref>{{cite journal | title = Tracking gene expression after DNA delivery using spatially indexed nanofiber arrays | author = [[Tim McKnight|McKnight, T.E.]], A.V. Melechko, D.K. Hensley, D.G.J. Mann, G.D. Griffin, and M.L. Simpson | journal = Nano Letters | date = 2004 | volume = 4| issue = 7 | pages = 1213–1219 | doi=10.1021/nl049504b| bibcode = 2004NanoL...4.1213M }}</ref> Needle-like [[nanostructure]]s are synthesized perpendicular to the surface of a [[Substrate (biology)|substrate]]. [[Plasmid]] [[DNA]] containing the gene, and intended for [[intracellular delivery]], is attached to the nanostructure surface. A chip with arrays of these needles is then pressed against cells or tissue. Cells that are impaled by nanostructures can express the delivered gene(s). |
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| ⚫ | [[Image:Green4.jpg|thumb|right|300px|Front: Scanning electron micrograph of |
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==Applications== |
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One of the features of impalefection is spatially resolved [[gene delivery]] that holds potential for such [[tissue engineering]] approaches in [[wound healing]] as [[gene activated matrix]] technology.<ref>{{Cite journal | author = Bonadio J. | date = 2000 | title = Local gene delivery for tissue regeneration | journal = E-biomed: The Journal of Regenerative Medicine | volume = 1| issue = 2 | pages = 25–29 | doi=10.1089/152489000414552}}</ref> Though impalefection is an efficient approach ''in vitro'', it has not yet been effectively used ''in vivo'' on live organisms and tissues.<ref>{{cite book|title=Nonviral Vectors for Gene Therapy: Physical Methods and Medical Translation|first1=Leaf|last1=Huang|first2=Dexi|last2=Liu|publisher=Academic Press|pages=7|date=2015}}</ref> |
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==Carrier materials== |
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Vertically aligned [[carbon nanofiber]] arrays prepared by [[photolithography]] and plasma enhanced chemical vapor deposition are one of the suitable types of material.<ref>{{cite journal|title=Transfer of Vertically Aligned Carbon Nanofibers to Polydimethylsiloxane (PDMS) While Maintaining their Alignment and Impalefection Functionality|first1=Ryan C. |last1=Pearce|first2= Justin G. |last2=Railsback|first3= Bryan D. |last3=Anderson|first4=Mehmet F. |last4=Sarac|first5= Timothy E. |last5=McKnight|first6= Joseph B. |last6=Tracy|first7=Anatoli V.|last7= Melechko|journal=ACS Applied Materials & Interfaces|volume=5|issue=3|date=2013|pages=878–882|doi=10.1021/am302501z |pmid=23281833 }}</ref> Silicon nanowires are another choice of [[nanoneedle]]s that have been utilized for impalefection. |
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1. Tracking gene expression after DNA delivery using spatially indexed nanofiber arrays |
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McKnight, T.E., A.V. Melechko, D.K. Hensley, D.G.J. Mann, G.D. Griffin, and M.L. Simpson, . |
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[[Image:Epitaxial Nanowire Heterostructures SEM image.jpg|thumb|300px|An [[Scanning electron microscope|SEM]] image of epitaxial nanowire heterostructures grown from catalytic gold nanoparticles.]] |
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Nano Letters, 2004. 4(7): p. 1213-1219 |
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== See also == |
== See also == |
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*[[Nanomedicine]] |
*[[Nanomedicine]] |
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*[[Tim McKnight]] |
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{{reflist}} |
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==External links== |
==External links== |
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*[http://nanobio.ftf.lth.se/~biokurs/projects/projects2006/Neuro_Louise.pdf#search=%22Impalefection%22 "An Attempted Nanowire Impalefection of Nerve Cells"] |
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*[http://tolik.freescience.org/publications/papers/paper29.htm "Intracellular integration of synthetic nanostructures with viable cells for controlled biochemical manipulation"] |
*[http://tolik.freescience.org/publications/papers/paper29.htm "Intracellular integration of synthetic nanostructures with viable cells for controlled biochemical manipulation"] |
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[[Category:Nanomaterials]] |
[[Category:Nanomaterials]] |
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[[Category:Laboratory techniques]] |
[[Category:Laboratory techniques]] |
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[[Category:Biotechnology]] |
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[[Category:Gene delivery]] |
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{{genetics-stub}} |
{{genetics-stub}} |
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Latest revision as of 08:53, 5 September 2022
Impalefection is a method of gene delivery using nanomaterials, such as carbon nanofibers, carbon nanotubes, nanowires.[1] Needle-like nanostructures are synthesized perpendicular to the surface of a substrate. Plasmid DNA containing the gene, and intended for intracellular delivery, is attached to the nanostructure surface. A chip with arrays of these needles is then pressed against cells or tissue. Cells that are impaled by nanostructures can express the delivered gene(s).
As one of the types of transfection, the term is derived from two words – impalement and infection.
Applications[edit]
One of the features of impalefection is spatially resolved gene delivery that holds potential for such tissue engineering approaches in wound healing as gene activated matrix technology.[2] Though impalefection is an efficient approach in vitro, it has not yet been effectively used in vivo on live organisms and tissues.[3]
Carrier materials[edit]
Vertically aligned carbon nanofiber arrays prepared by photolithography and plasma enhanced chemical vapor deposition are one of the suitable types of material.[4] Silicon nanowires are another choice of nanoneedles that have been utilized for impalefection.
See also[edit]
References[edit]
- ^ McKnight, T.E., A.V. Melechko, D.K. Hensley, D.G.J. Mann, G.D. Griffin, and M.L. Simpson (2004). "Tracking gene expression after DNA delivery using spatially indexed nanofiber arrays". Nano Letters. 4 (7): 1213–1219. Bibcode:2004NanoL...4.1213M. doi:10.1021/nl049504b.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Bonadio J. (2000). "Local gene delivery for tissue regeneration". E-biomed: The Journal of Regenerative Medicine. 1 (2): 25–29. doi:10.1089/152489000414552.
- ^ Huang, Leaf; Liu, Dexi (2015). Nonviral Vectors for Gene Therapy: Physical Methods and Medical Translation. Academic Press. p. 7.
- ^ Pearce, Ryan C.; Railsback, Justin G.; Anderson, Bryan D.; Sarac, Mehmet F.; McKnight, Timothy E.; Tracy, Joseph B.; Melechko, Anatoli V. (2013). "Transfer of Vertically Aligned Carbon Nanofibers to Polydimethylsiloxane (PDMS) While Maintaining their Alignment and Impalefection Functionality". ACS Applied Materials & Interfaces. 5 (3): 878–882. doi:10.1021/am302501z. PMID 23281833.
External links[edit]