Amido black 10B

Names
IUPAC name 4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6- -(phenylazo)-2,7-Naphthalene disulfonic acid, disodium salt
Other names Amidoschwarz Naphthol blue black Acid Black 1 Acidal Black 10B Acidal Navy Blue 3BR Naphthalene Black 10B (see talk page) Buffalo Black NBR C.I. 20470
Identifiers
CAS Number	1064-48-8 Y
3D model (JSmol)	Interactive image
ChEBI	CHEBI:86230 N
ChemSpider	10660833 Y
ECHA InfoCard	100.012.640
PubChem CID	9566044
CompTox Dashboard (EPA)	DTXSID1024415
InChI InChI=1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;; Y Key: AOMZHDJXSYHPKS-DROYEMJCSA-L Y InChI=1/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;; Key: AOMZHDJXSYHPKS-NJAWIMKDBA
SMILES [Na+].[Na+].O=N(=O)c1ccc(cc1)/N=N/c2c(N)c4c(cc2S([O-])(=O)=O)cc(c(/N=N/c3ccccc3)c4O)S([O-])(=O)=O
Properties
Chemical formula	C22H14N6Na2O9S2
Molar mass	616.49 g·mol−1
Appearance	Dark red to black
Solubility in water	~ 30 g/l at 20 °C (68 °F)
Hazards
NFPA 704 (fire diamond)	2 0 0
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). N verify (what is YN ?) Infobox references

Amido black 10B is an amino acid staining diazo dye used in biochemical research to stain for total protein on transferred membrane blots. It is also used in criminal investigations to detect blood present with latent fingerprints. It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.

External links

• www.chemicalland21.com
• MSDS at Oxford University