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| ImageFile = Amido Black.svg
| ImageFile = Amido Black.svg
| ImageSize = 320px
| ImageSize = 320px
| ImageFile1 = Amido-black-10B-sodium-3D-spacefill.png
| ImageSize1 = 280
| ImageAlt1 = Space-filling model of the amido black 10B molecule
| IUPACName = <nowiki>4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6- -(phenylazo)-2,7-Naphthalene disulfonic acid, disodium salt</nowiki>
| IUPACName = <nowiki>4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6- -(phenylazo)-2,7-Naphthalene disulfonic acid, disodium salt</nowiki>
| OtherNames = Amidoschwarz <br /> Naphthol blue black <br /> Acid Black 1 <br /> Acidal Black 10B <br /> Acidal Navy Blue 3BR <br /> Naphthalene Black 10B (see [[Talk:Amido black 10B|talk page]]) <br /> Buffalo Black NBR <br /> C.I. 20470
| OtherNames = Amidoschwarz <br /> Naphthol blue black <br /> Acid Black 1 <br /> Acidal Black 10B <br /> Acidal Navy Blue 3BR <br /> Naphthalene Black 10B (see [[Talk:Amido black 10B|talk page]]) <br /> Buffalo Black NBR <br /> C.I. 20470

Revision as of 12:22, 18 May 2015

Amido black 10B
Space-filling model of the amido black 10B molecule
Names
IUPAC name
4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6- -(phenylazo)-2,7-Naphthalene disulfonic acid, disodium salt
Other names
Amidoschwarz
Naphthol blue black
Acid Black 1
Acidal Black 10B
Acidal Navy Blue 3BR
Naphthalene Black 10B (see talk page)
Buffalo Black NBR
C.I. 20470
Identifiers
3D model (JSmol)
ChemSpider
ECHA InfoCard 100.012.640 Edit this at Wikidata
  • InChI=1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;; checkY
    Key: AOMZHDJXSYHPKS-DROYEMJCSA-L checkY
  • InChI=1/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
    Key: AOMZHDJXSYHPKS-NJAWIMKDBA
  • [Na+].[Na+].O=N(=O)c1ccc(cc1)/N=N/c2c(N)c4c(cc2S([O-])(=O)=O)cc(c(/N=N/c3ccccc3)c4O)S([O-])(=O)=O
Properties
C22H14N6Na2O9S2
Molar mass 616.49 g·mol−1
Appearance Dark red to black
~ 30 g/l at 20 °C (68 °F)
Hazards
NFPA 704 (fire diamond)
NFPA 704 four-colored diamondHealth 2: Intense or continued but not chronic exposure could cause temporary incapacitation or possible residual injury. E.g. chloroformFlammability 0: Will not burn. E.g. waterInstability 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g. liquid nitrogenSpecial hazards (white): no code
2
0
0
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
checkY verify (what is checkY☒N ?)

Amido black 10B is an amino acid staining diazo dye used in biochemical research to stain for total protein on transferred membrane blots. It is also used in criminal investigations to detect blood present with latent fingerprints. It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.

External links

source: https://en.wikipedia.org/w/index.php?title=Amido_black_10B&diff=prev&oldid=662926583

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