THC Science

Amido black 10B^[1]
Names
	IUPAC name Sodium 4-amino-5-hydroxy-3-((E)-(4-nitrophenyl)diazenyl)-6-((E)-phenyldiazenyl)naphthalene-2,7-disulfonate
	Other names 4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-2,7-naphthalene disulfonic acid, disodium salt; Amidoschwarz; Naphthol blue black; Acid Black 1; Acidal Black 10B; Acidal Navy Blue 3BR; Naphthalene Black 10B ; Buffalo Black NBR; C.I. 20470
Identifiers
CAS Number	1064-48-8 ;
3D model (JSmol)	Interactive image;
ChEBI	CHEBI:86230 ;
ChemSpider	10660833 ;
ECHA InfoCard	100.012.640
EC Number	213-903-1;
PubChem CID	9566044;
UNII	SZT789770M ;
CompTox Dashboard (EPA)	DTXSID1024415 ;
	InChI InChI=1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2+1/p-2/b26-25+,27-24+;; Key: AOMZHDJXSYHPKS-DROYEMJCSA-L ; InChI=1/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2+1/p-2/b26-25+,27-24+;; Key: AOMZHDJXSYHPKS-NJAWIMKDBA;
	SMILES [Na+].[Na+].O=N(=O)c1ccc(cc1)/N=N/c2c(N)c4c(cc2S([O-])(=O)=O)cc(c(/N=N/c3ccccc3)c4O)S([O-])(=O)=O;
Properties
Chemical formula	C22H14N6Na2O9S2
Molar mass	616.49 g·mol−1
Appearance	Dark red to black
Solubility in water	~ 30 g/L at 20 °C (68 °F)
Hazards
	GHS labelling:
Pictograms
Signal word	Warning
NFPA 704 (fire diamond)	2 0 0
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). verify (what is ?) Infobox references

Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, such as the western blot.^[2] It is also used in criminal investigations to detect blood present with latent fingerprints - it stains the proteins in blood a blue-black color.^[3] Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.

References[edit]

^ "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017.
^ Kurien, B. T., & Scofield, R. H. (2015). Western Blotting: An Introduction. In B. T. Kurien & R. H. Scofield (Eds.), Western Blotting: Methods and Protocols (pp. 17–30). Springer. ISBN 978-1-4939-2694-7
^ Bossers, L. C. A. M., Roux, C., Bell, M., & McDonagh, A. M. (2011). Methods for the enhancement of fingermarks in blood. Forensic Science International, 210(1), 1–11. doi:10.1016/j.forsciint.2011.04.006

External links[edit]

MSDS at Oxford University

[1] "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017.

[2] Kurien, B. T., & Scofield, R. H. (2015). Western Blotting: An Introduction. In B. T. Kurien & R. H. Scofield (Eds.), Western Blotting: Methods and Protocols (pp. 17–30). Springer. ISBN 978-1-4939-2694-7

[3] Bossers, L. C. A. M., Roux, C., Bell, M., & McDonagh, A. M. (2011). Methods for the enhancement of fingermarks in blood. Forensic Science International, 210(1), 1–11. doi:10.1016/j.forsciint.2011.04.006

[1]

[2]

[3]

@@ Line 3: / Line 3: @@
 | Watchedfields = changed
 | verifiedrevid = 401248657
-| ImageFile = Amido Black.svg
+| ImageFile = Amido black new.svg
 | ImageSize = 320px
+| Reference = <ref>{{cite web | url = http://www.chemicalland21.com/specialtychem/finechem/AMIDO%20BLACK%2010B.htm | title = AMIDO BLACK 10B | publisher = chemicalland21.com | accessdate = December 2, 2017}}</ref><!-- synonym list is derived from this reference -->
-| ImageFile1 = Amido-black-10B-sodium-3D-spacefill.png
+| IUPACName = Sodium 4-amino-5-hydroxy-3-((''E'')-(4-nitrophenyl)diazenyl)-6-((''E'')-phenyldiazenyl)naphthalene-2,7-disulfonate
-| ImageSize1 = 280
+| OtherNames = 4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-2,7-naphthalene disulfonic acid, disodium salt<br />Amidoschwarz<br />Naphthol blue black<br />Acid Black 1<br />Acidal Black 10B<br />Acidal Navy Blue 3BR<br />Naphthalene Black 10B <br />Buffalo Black NBR<br />C.I. 20470
-| ImageAlt1 = Space-filling model of the amido black 10B molecule
-| IUPACName = <nowiki>4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6- -(phenylazo)-2,7-Naphthalene disulfonic acid, disodium salt</nowiki>
-| OtherNames = Amidoschwarz <br /> Naphthol blue black <br /> Acid Black 1 <br /> Acidal Black 10B <br /> Acidal Navy Blue 3BR <br /> Naphthalene Black 10B (see [[Talk:Amido black 10B|talk page]]) <br /> Buffalo Black NBR <br /> C.I. 20470
 |Section1={{Chembox Identifiers
 | InChI = 1/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
@@ Line 19: / Line 17: @@
 | CASNo_Ref = {{cascite|correct|??}}
 | CASNo = 1064-48-8
+| UNII_Ref = {{fdacite|changed|FDA}}
+| UNII = SZT789770M
 | PubChem = 9566044
+| EINECS = 213-903-1
 | ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}}
 | ChemSpiderID = 10660833
-| ChEBI_Ref = {{ebicite|changed|EBI}}
+| ChEBI_Ref = {{ebicite|correct|EBI}}
 | ChEBI = 86230
 | SMILES = [Na+].[Na+].O=N(=O)c1ccc(cc1)/N=N/c2c(N)c4c(cc2S([O-])(=O)=O)cc(c(/N=N/c3ccccc3)c4O)S([O-])(=O)=O
@@ Line 32: / Line 33: @@
 | MeltingPt =
 | BoilingPt =
-| Solubility = ~ 30 g/l at {{convert|20|C}}
+| Solubility = ~ 30 g/L at {{convert|20|C}}
   }}
 |Section7={{Chembox Hazards
@@ Line 41: / Line 42: @@
 | NFPA-F = 0
 | NFPA-R = 0
-| RPhrases = {{R20}}, {{R21}}, {{R33}}
+| GHSPictograms = {{GHS07}}
+| GHSSignalWord = Warning
-| SPhrases = {{S28}}A, {{S37}}, {{S45}}
+| HPhrases =
+| PPhrases =
   }}
 }}
-'''Amido black 10B''' is an [[amino acid]] [[staining]] [[diazo dye]] used in biochemical research to stain for total [[protein]] on transferred membrane blots.  It is also used in criminal investigations to detect [[blood]] present with latent [[fingerprint]]s. It stains the proteins in blood a blue-black color.  Amido Black can be either [[methanol]] or water based as it readily dissolves in both. With [[picric acid]], in a [[van Gieson]] procedure, it can be used to stain [[collagen]] and [[reticulin]].
+'''Amido black 10B''' is an [[amino acid]] [[staining]] [[azo dye]] used in biochemical research to stain for total [[protein]] on transferred membrane blots, such as the [[western blot]].<ref>Kurien, B. T., & Scofield, R. H. (2015). Western Blotting: An Introduction. In B. T. Kurien & R. H. Scofield (Eds.), Western Blotting: Methods and Protocols (pp. 17–30). Springer. {{ISBN|978-1-4939-2694-7}}</ref> It is also used in criminal investigations to detect [[blood]] present with latent [[fingerprint]]s - it stains the proteins in blood a blue-black color.<ref>Bossers, L. C. A. M., Roux, C., Bell, M., & McDonagh, A. M. (2011). Methods for the enhancement of fingermarks in blood. Forensic Science International, 210(1), 1–11. {{doi|10.1016/j.forsciint.2011.04.006}}</ref> Amido Black can be either [[methanol]] or water based as it readily dissolves in both. With [[picric acid]], in a [[van Gieson]] procedure, it can be used to stain [[collagen]] and [[reticulin]].
+==See also==
+*[[Western blot normalization]]
+==References==
+{{reflist}}
 ==External links==
+*[https://web.archive.org/web/20071011133532/http://ptcl.chem.ox.ac.uk/MSDS/AM/amido_black_10B.html MSDS at Oxford University]
-*[http://www.chemicalland21.com/specialtychem/finechem/AMIDO%20BLACK%2010B.htm www.chemicalland21.com]
-*[http://ptcl.chem.ox.ac.uk/MSDS/AM/amido_black_10B.html MSDS at Oxford University]
+{{Glutamate metabolism and transport modulators}}
 {{DEFAULTSORT:Amido Black 10b}}
 [[Category:Azo dyes]]
 [[Category:Naphthalenesulfonates]]
-[[Category:Sodium compounds]]
+[[Category:Organic sodium salts]]
 [[Category:1-Naphthols]]
-[[Category:Nitrobenzenes]]
+[[Category:Nitrobenzene derivatives]]
 [[Category:Aromatic amines]]
+[[Category:Acid dyes]]

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Latest revision as of 04:46, 9 April 2024

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