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Michael7604 (talk | contribs) change category from Nitrobenzenes to Nitroarenes |
203.153.198.190 (talk) Added relevant citations in the field for each of the two major use-case claims. They were correct, but uncited, and this compound is niche enough that proper citations are probably for the best. |
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'''Amido black 10B''' is an [[amino acid]] [[staining]] [[azo dye]] used in biochemical research to stain for total [[protein]] on transferred membrane blots, such as the [[western blot]]. It is also used in criminal investigations to detect [[blood]] present with latent [[fingerprint]]s - it stains the proteins in blood a blue-black color. Amido Black can be either [[methanol]] or water based as it readily dissolves in both. With [[picric acid]], in a [[van Gieson]] procedure, it can be used to stain [[collagen]] and [[reticulin]]. |
'''Amido black 10B''' is an [[amino acid]] [[staining]] [[azo dye]] used in biochemical research to stain for total [[protein]] on transferred membrane blots, such as the [[western blot]].<ref>Kurien, B. T., & Scofield, R. H. (2015). Western Blotting: An Introduction. In B. T. Kurien & R. H. Scofield (Eds.), Western Blotting: Methods and Protocols (pp. 17–30). Springer. {{ISBN|978-1-4939-2694-7}}</ref> It is also used in criminal investigations to detect [[blood]] present with latent [[fingerprint]]s - it stains the proteins in blood a blue-black color.<ref>Bossers, L. C. A. M., Roux, C., Bell, M., & McDonagh, A. M. (2011). Methods for the enhancement of fingermarks in blood. Forensic Science International, 210(1), 1–11. {{doi|10.1016/j.forsciint.2011.04.006}}</ref> Amido Black can be either [[methanol]] or water based as it readily dissolves in both. With [[picric acid]], in a [[van Gieson]] procedure, it can be used to stain [[collagen]] and [[reticulin]]. |
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==See also== |
==See also== |
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[[Category:Organic sodium salts]] |
[[Category:Organic sodium salts]] |
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[[Category:1-Naphthols]] |
[[Category:1-Naphthols]] |
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[[Category: |
[[Category:Nitrobenzene derivatives]] |
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[[Category:Aromatic amines]] |
[[Category:Aromatic amines]] |
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[[Category:Acid dyes]] |
[[Category:Acid dyes]] |
Latest revision as of 04:46, 9 April 2024
Names | |
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IUPAC name
Sodium 4-amino-5-hydroxy-3-((E)-(4-nitrophenyl)diazenyl)-6-((E)-phenyldiazenyl)naphthalene-2,7-disulfonate
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Other names
4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-2,7-naphthalene disulfonic acid, disodium salt
Amidoschwarz Naphthol blue black Acid Black 1 Acidal Black 10B Acidal Navy Blue 3BR Naphthalene Black 10B Buffalo Black NBR C.I. 20470 | |
Identifiers | |
3D model (JSmol)
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ChEBI | |
ChemSpider | |
ECHA InfoCard | 100.012.640 |
EC Number |
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PubChem CID
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UNII | |
CompTox Dashboard (EPA)
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Properties | |
C22H14N6Na2O9S2 | |
Molar mass | 616.49 g·mol−1 |
Appearance | Dark red to black |
~ 30 g/L at 20 °C (68 °F) | |
Hazards | |
GHS labelling: | |
Warning | |
NFPA 704 (fire diamond) | |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, such as the western blot.[2] It is also used in criminal investigations to detect blood present with latent fingerprints - it stains the proteins in blood a blue-black color.[3] Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.
See also[edit]
References[edit]
- ^ "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017.
- ^ Kurien, B. T., & Scofield, R. H. (2015). Western Blotting: An Introduction. In B. T. Kurien & R. H. Scofield (Eds.), Western Blotting: Methods and Protocols (pp. 17–30). Springer. ISBN 978-1-4939-2694-7
- ^ Bossers, L. C. A. M., Roux, C., Bell, M., & McDonagh, A. M. (2011). Methods for the enhancement of fingermarks in blood. Forensic Science International, 210(1), 1–11. doi:10.1016/j.forsciint.2011.04.006