Holy Rood Church may refer to:

• Holy Rood Church, Barnsley, South Yorkshire
• Holy Rood Church, Market Rasen, Lincolnshire
• Holy Rood Church, Ossington, Nottinghamshire
• Holy Rood Church, Swindon, Wiltshire
• Holy Rood Church, Watford, Hertfordshire
• Church of the Holy Rood, Edwalton, Nottinghamshire
• Church of the Holy Rude, Stirling
• Holyrood Church, Southampton, Hampshire
• Holyrood Abbey Church, Edinburgh
• Holyrood Abbey, Edinburgh

• Holyrood Episcopal Church, New York City

• Holyrood (disambiguation)