Woolston may refer to:

• Woolston, Cheshire, a village and civil parish in Warrington
• Woolston, Devon, on the list of United Kingdom locations: Woof-Wy near Kingsbridge, Devon
• Woolston, Southampton, a city suburb in Hampshire
• Some hamlets:
  • Woolston, Cornwall, to the northwest of St Ive
  • Woolston, north Shropshire, near Oswestry
  • Woolston, south Shropshire, near Church Stretton and Craven Arms
  • Woolston, Somerset, near the village of North Cadbury, between Wincanton and Yeovil

• Woolston, New Zealand, a suburb of Christchurch

• Andrew Woolston, English curler in the 2010–2015 European Curling Championships
• Beulah Woolston (1828–1886), pioneering American missionary teacher in China
• Bob Woolston (born 1968), English cricketer
• Thomas Woolston (1668–1733), English theologian
• Thomas G. Woolston (fl. 1995), American patent attorney
• Florence Guy Woolston Seabury (1881–1951), suffragist, journalist in New York

• Woolston Rovers, a rugby league team based in Warrington
• Woolston Technical, a semi-professional association football club based in Woolston, New Zealand
• Woolston W.M.C., an association football club based in Woolston, New Zealand
• Woolston Works F.C., a defunct late 19th century football club formerly based at Woolston, Hampshire

• HMS Woolston (1918) (L49), a W Class destroyer of the Royal Navy
• Woolston Memorial Hospital, a Christian hospital established in 19th century Fuzhou
• Woolston railway station, serving Woolston, Southampton
• Woolston School, a secondary comprehensive school in Southampton, Hampshire, in southern England
• Woolston-Steen Theological Seminary, a college in Index, Washington, U.S., offering degrees in Wiccan Ministry.

• Woolaston, a village in the Forest of Dean, Gloucestershire, England
• Woollaston (disambiguation), a disambiguation page
• Wolston, Warwickshire