Madonna may refer to:

• The Madonna, Roman Catholic title for Mary, mother of Jesus
• Madonna (art), a medieval Italian term for a noble or otherwise important woman

People:

• Madonna (entertainer), an American pop singer and actress
• Madonna Wayne Gacy, the current keyboard player for Marilyn Manson

Places

• Madona, Latvia, a town in Latvia
• House of the Black Madonna, a cubist building in Prague, Czech republic
• Madonna del Ghisallo, a hill close to Lake Como in Italy
• Madonna Inn, a motel of flamboyant style in San Luis Obispo, California
• Madonna of the Trail, a series of historical monuments along the National Old Trails Highway, from Maryland to California
• Madonna Oriente, a Moon goddess that was worshipped in a cult that developed in Milan towards the end of the 14th century
• Madonna University, a private, non-profit, Catholic Franciscan college located in Livonia, Michigan

Other

• Madonna lily, a plant in the genus Lilium, a "true lily", that is native to Asia Minor
• Black Madonna, a statue or painting of Mary in which she is depicted with black skin
  • A song of the same name by Noe Venable
• Madonna (skateboarding trick)
• Madonna (Madonna album), a 1983 album by the American pop singer of the same name
• Madonna (Trail of Dead), a 1999 album by the band …And You Will Know Us by the Trail of Dead
• Lady Madonna, a song by The Beatles
• Madonna, a painting by Edvard Munch