Pioneer Conference may refer to:

• Pioneer Conference (Illinois), intercollegiate athletic conference that existed in the U.S. state of Illinois from 1947 to 1949
• Pioneer Conference (Indiana), high school athletic conference in the U.S. state of Indiana, founded in 2009
• Pioneer Conference (Ohio), high school athletic conference in the U.S. state of Ohio, founded in 1977
• Pioneer Conference (Texas), junior college athletic conference that existed in the U.S. state of Texas from 1949 to 1961