The following highways are numbered 427:

Canada[edit]

• Manitoba Provincial Road 427
• Ontario Highway 427

India[edit]

• National Highway 427 (India)

Japan[edit]

• Japan National Route 427

United States[edit]

• County Road 427 (Seminole County, Florida)
• Indiana State Road 427
  • County Road 427 (DeKalb County, Indiana)
• Nevada State Route 427
• New York State Route 427
  • New York State Route 427 (former)
• Pennsylvania Route 427
• Puerto Rico Highway 427
• Texas State Highway Loop 427 (former)