The following highways are numbered 254:

• Manitoba Provincial Road 254
• Prince Edward Island Route 254

• National Route 254

• R254 regional road

• Japan National Route 254

• Arkansas Highway 254
• California State Route 254
• Connecticut Route 254
• Georgia State Route 254
• K-254 (Kansas highway)
• Kentucky Route 254
• Maryland Route 254
• Minnesota State Highway 254
• Missouri Route 254
• Montana Secondary Highway 254
• New Mexico State Road 254
• New York State Route 254
• Ohio State Route 254
• Pennsylvania Route 254
• South Carolina Highway 254
• Tennessee State Route 254
• Texas State Highway 254
  • Texas State Highway Loop 254
  • Farm to Market Road 254 (Texas)
• Utah State Route 254 (former)
• Virginia State Route 254
• Wyoming Highway 254