The following highways are numbered 227:

• Manitoba Provincial Road 227
• Prince Edward Island Route 227
• Quebec Route 227

• China National Highway 227

• National Route 227

• National Highway 227 (India)

• Route 227 (Israel)

• Japan National Route 227

• U.S. Route 227 (former)
• Alabama State Route 227
• California State Route 227
• Colorado State Highway 227
• Florida State Road 227 (former)
• Georgia State Route 227
• Indiana State Road 227
• Kentucky Route 227
• Maine State Route 227
• Maryland Route 227
• M-227 (Michigan highway)
• Minnesota State Highway 227
• Montana Secondary Highway 227
• Nevada State Route 227
• New York State Route 227
• Oregon Route 227
• Pennsylvania Route 227
• Tennessee State Route 227
• Texas State Highway 227 (former)
• Utah State Route 227
• Virginia State Route 227