Protoculture may refer to:

• Protoculture, the passing of learned behaviors from one generation to another in non-human primates
• A term used in the science fiction anime series The Super Dimension Fortress Macross, but referring to different things depending on the version:
  • In Macross: an ancient race of alien humanoids
  • In the American adaptation, Robotech: an ancient power source and foodstuff
• Protoculture Addicts, a North American anime and manga magazine
  • Protoculture Inc., publisher of the magazine
• "Proto Culture", a hip-hop song about video games, by artist Del tha Funkee Homosapien (featuring Khaos Unique), on the album Both Sides of the Brain