Zero One may refer to:
- Zero 1 (band), a band
- "Zero One" (song), a 2018 song by K?d
- "Zero-One", a song by Northlane from the album Mesmer, 2017
- Pro Wrestling Zero1, a Japanese pro wrestling promotion
- Zero-One, the name of the vehicle in the Pokémon Snap video game
- Zero One, the name of the computer-controlled city in the Matrix trilogy
- Kamen Rider Zero-One, a 2019–20 Japanese tokusatsu series
- Zero One (TV series), a 1962-65 British TV series
- Zero One (album), a 2020 album by the Living Tombstone
- Zero One, a manga written by Hiroya Oku
- Zero One, an adventure video game published by Fuuki