John Crowley may refer to:

• John Crowley (author) (born 1942), American author
• John Crowley (baseball) (1862–1896), American Major League catcher
• John Crowley (biotech executive) (born 1967), American biotechnology executive
• John Crowley (bishop) (born 1941), former bishop of Middlesbrough
• John Crowley (director) (born 1969), Irish theatre and film director
• John Crowley (politician) (1870–1934), Irish Sinn Féin politician
• John Crowley (1659–1728), British politician
• John Francis Crowley (1891–1942), Irish revolutionary and hunger striker
• John Powers Crowley (1936–1989), U.S. federal judge
• Johnny Crowley (born 1956), Irish hurler
• Johnny Crowley (Gaelic footballer), Gaelic footballer with Kerry GAA
• John J. Crowley (1891-1940), American Catholic priest