Ann Walker or Anne Walker may refer to:
- Ann Walker (landowner) (1803–1854), Yorkshire landowner, partner of Anne Lister
- Anne Walker (architectural historian) (born 1973), American architectural historian and author
- Anne Walker (artist) (born 1933), painter and printmaker
- Anne Walker (astronomer) (1863–1940), British astronomer
- Anne Weightman Walker (1844–1932), American philanthropist