Palestrina is a city in Lazio, Italy.
Palestrina may also refer to:
- 4850 Palestrina, a minor planet
- Palestrina (opera), a 1917 opera by Hans Pfitzner
- Palestrina - Prince of Music, a 2009 Italian/German music film
- Palestrina, an opera by Johann Sachs
- Palestrina Glacier, Alexander Island, Antarctica
- Giovanni Pierluigi da Palestrina, an Italian Renaissance composer
- Roman Catholic Suburbicarian Diocese of Palestrina, based in the city
- U.S. Palestrina 1919, an Italian association football club