Authors
Eva Nogales, Sjors HW Scheres
Publication date
2015/5/21
Source
Molecular cell
Volume
58
Issue
4
Pages
677-689
Publisher
Elsevier
Description
3D cryo-electron microscopy (cryo-EM) is an expanding structural biology technique that has recently undergone a quantum leap progression in its achievable resolution and its applicability to the study of challenging biological systems. Because crystallization is not required, only small amounts of sample are needed, and because images can be classified in a computer, the technique has the potential to deal with compositional and conformational mixtures. Therefore, cryo-EM can be used to investigate complete and fully functional macromolecular complexes in different functional states, providing a richness of biological insight. In this review, we underlie some of the principles behind the cryo-EM methodology of single particle analysis and discuss some recent results of its application to challenging systems of paramount biological importance. We place special emphasis on new methodological developments …
Scholar articles
E Nogales, SHW Scheres - Molecular cell, 2015