Benedictine may mean:

• A Benedictine, a monk or nun who belongs to the Order of Saint Benedict
• Benedictine abbey
• Benedictine College in Atchison, Kansas
• Benedictine High School (disambiguation)
• Benedictine University in Lisle, Illinois
• Bénédictine, a liqueur
• Benedictine (spread), a spread/dip made with cucumbers and cream cheese