Staff may refer to:

• Walking staff, an instrument used for balance when walking
• Staff, a weapon used in stick-fighting
  • Quarterstaff, a European pole weapon
• Staff of office, a pole that indicates a position
• Staff (railway signalling), a token authorizing a locomotive driver to use a particular stretch of single track
• Level staff, also called levelling rod, a graduated rod for comparing heights
• Fire staff, a staff of wood or metal and Kevlar, used for fire dancing and performance
• Flagstaff, on which a flag is flown
• Scout staff, a shoulder-high pole traditionally carried by Boy Scouts, for various uses in emergencies
• Pilgrim's staff, a walking stick used by pilgrims during their pilgrimages

• Staff (military), the organ of military command and planning
• USS Staff (AM-114), a United States Navy minesweeper
• Smart Target-Activated Fire and Forget (XM943 STAFF), an American-made experimental 120×570mm NATO tank gun shell

• Staff (name), a list of people with either the surname or nickname

• People in employment within any organization
• Staff (music), a set of five horizontal lines upon which notes are placed in written music notation
• Staff (building material) (short for staffieren), an artificial stone product used as ornament
• Jack Staff, British superhero
• Staffordshire, a county in England
• Staff, a nickname for the Staffordshire Bull Terrier

• Celastrus, a shrub genus commonly known as the staff vine or staff tree
• Staphylococcus, often shortened to "staph", bacteria which can cause infection