Cannabaceae

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4-oxalocrotonate tautomerase
Pseudomonas putida 4-oxalocrotonate tautomerase hexamer bound to 2-oxo-3-pentenoic acid. PDB: 1BJP​
Identifiers
SymbolTaut
PfamPF01361
InterProIPR004370
CDDcd00491
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary
PDBPDB: 1bjp​ PDB: 1gyj​ PDB: 1gyx​ PDB: 1gyy​ PDB: 1otf​ PDB: 1s0y​ PDB: 4ota​ PDB: 4otb​ PDB: 4otc​

4-Oxalocrotonate tautomerase (EC 5.3.2.6) or 4-OT is an enzyme that converts 2-hydroxymuconate to the αβ-unsaturated ketone, 2-oxo-3-hexenedioate.[1] This enzyme forms part of a bacterial metabolic pathway that oxidatively catabolizes toluene, o-xylene, 3-ethyltoluene, and 1,2,4-trimethylbenzene into intermediates of the citric acid cycle. With a monomer size of just 62 amino acid residues, the 4-Oxalocrotonate tautomerase is one of the smallest enzyme subunits known.[2] However, in solution, the enzyme forms a hexamer of six identical subunits, so the active site may be formed by amino acid residues from several subunits.[3] This enzyme is also unusual in that it uses a proline residue at the amino terminus as an active site residue.

Reaction catalyzed by 4-oxalocrotonate tautomerase.[1]

References[edit]

  1. ^ a b Chen LH, Kenyon GL, Curtin F, Harayama S, Bembenek ME, Hajipour G, Whitman CP (September 1992). "4-Oxalocrotonate tautomerase, an enzyme composed of 62 amino acid residues per monomer". The Journal of Biological Chemistry. 267 (25): 17716–21. PMID 1339435.
  2. ^ Whitman CP (June 2002). "The 4-oxalocrotonate tautomerase family of enzymes: how nature makes new enzymes using a beta-alpha-beta structural motif". Archives of Biochemistry and Biophysics. 402 (1): 1–13. doi:10.1016/S0003-9861(02)00052-8. PMID 12051677.
  3. ^ Subramanya HS, Roper DI, Dauter Z, Dodson EJ, Davies GJ, Wilson KS, Wigley DB (January 1996). "Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases". Biochemistry. 35 (3): 792–802. doi:10.1021/bi951732k. PMID 8547259.


source: https://en.wikipedia.org/wiki/4-Oxalocrotonate_tautomerase

One thought on “Cannabaceae”

  1. Well, that’s interesting to know that Psilotum nudum are known as whisk ferns. Psilotum nudum is the commoner species of the two. While the P. flaccidum is a rare species and is found in the tropical islands. Both the species are usually epiphytic in habit and grow upon tree ferns. These species may also be terrestrial and grow in humus or in the crevices of the rocks.
    View the detailed Guide of Psilotum nudum: Detailed Study Of Psilotum Nudum (Whisk Fern), Classification, Anatomy, Reproduction

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