MMN medium or Modified Melin-Norkrans medium is a type of agar growth medium, used to grow cultures of mycorrhizal fungi, such as Boletus edulis and Tricholoma matsutake.[1][2][3][4][5] It was first described by DH. Marx in The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria in 1969.[1] The acidic pH (5.6) of MMN agar inhibits bacterial growth.[6]
Typical composition
[edit]MMN agar typically contains:[6]
- 10 g/L glucose
- 3 g/L malt extract
- 0.25 g/L (NH4)2HPO4
- 0.025 g/L NaCl
- 0.5 g/L KH2PO4
- 0.05 g/L CaCl2
- 0.15 g/L MgSO4.7 H2O
- 0.012g/L FeCl3. 6 H2O
- 0.003 g/L thiamine
- 15 g/L agar
References
[edit]- ^ a b Águeda, Beatriz; Parladé, Javier; Fernández-Toirán, Luz Marina; Cisneros, Óscar; de Miguel, Ana María; Modrego, María Pilar; Martínez-Peña, Fernando; Pera, Joan (October 2008). "Mycorrhizal synthesis between Boletus edulis species complex and rockroses (Cistus sp.)". Mycorrhiza. 18 (8): 443–449. doi:10.1007/s00572-008-0192-3. ISSN 0940-6360. PMID 18695982. S2CID 11233267.
- ^ Mediavilla, Olaya; Olaizola, Jaime; Santos-del-Blanco, Luis; Oria-de-Rueda, Juan Andrés; Martín-Pinto, Pablo (February 2016). "Mycorrhization between Cistus ladanifer L. and Boletus edulis Bull is enhanced by the mycorrhiza helper bacteria Pseudomonas fluorescens Migula". Mycorrhiza. 26 (2): 161–168. doi:10.1007/s00572-015-0657-0. ISSN 0940-6360. PMID 26208816. S2CID 254061088.
- ^ Islam, Ferzana; Ohga, Shoji (2013-03-12). "Effects of Media Formulation on the Growth and Morphology of Ectomycorrhizae and Their Association with Host Plant". International Scholarly Research Notices. 2013: e317903. doi:10.1155/2013/317903.
- ^ Chaitali Bhattacharya; Alok Adholeya. "Showcasing Ectomycorrhizal Culture". bookstore.teri.res.in. Retrieved 2023-02-04.
- ^ Declerck, Stéphane; Strullu, D. G.; Fortin, André (2005-04-13). In Vitro Culture of Mycorrhizas. Springer Science & Business Media. p. 259. ISBN 978-3-540-24027-3.
- ^ a b "CBS List of Media" (PDF).
Well, that’s interesting to know that Psilotum nudum are known as whisk ferns. Psilotum nudum is the commoner species of the two. While the P. flaccidum is a rare species and is found in the tropical islands. Both the species are usually epiphytic in habit and grow upon tree ferns. These species may also be terrestrial and grow in humus or in the crevices of the rocks.
View the detailed Guide of Psilotum nudum: Detailed Study Of Psilotum Nudum (Whisk Fern), Classification, Anatomy, Reproduction