Middlesbrough is a large town, in the north east of England.

Middlesbrough and its variants Middlesborough, Middlesboro and Middlesbro may also refer to:

• Middlesboro, Kentucky, US, former spelling was Middlesborough
• Borough of Middlesbrough, a district of North Yorkshire
• Middlesbrough Rural District, in the North Riding of Yorkshire, England, from 1894 to 1932
• Middlesbrough railway station, serving the town of Middlesbrough, England

• Middlesboro crater, a Permian period meteorite crater in Kentucky, United States
• Middlesbrough meteorite, a meteorite which fell in Middlesbrough, England, in 1881

• Middlesbrough F.C., an English association football club
• Middlesbrough Futsal Club, an English futsal club
• Middlesbrough Cricket Club, an English cricket club
• Middlesbrough W.F.C., an English association football club
• Middlesbrough RUFC, an English rugby union football club
• Middlesbrough Bears, an English speedway team from 1939 until 1996
• Middlesbrough Ironopolis F.C., an English association football club from 1889 to 1894

• Middlesbrough (UK Parliament constituency), a borough constituency in the House of Commons
• Middlesbrough South and East Cleveland (UK Parliament constituency), a constituency in the House of Commons
• Middlesbrough East (UK Parliament constituency), a former parliamentary constituency
• Middlesbrough West (UK Parliament constituency), a former parliamentary constituency

• Middlesbrough during World War II
• Middleborough, Massachusetts