A major topic of debate in Old World prehistory is the relative importance of population movement versus cultural diffusion in explaining the spread of agriculture into and across Europe following its inception in southwestern Asia. An important set of data that has surprisingly been largely absent from this debate is the preserved crops and associated weeds of the earliest farmers. An analysis of archaeobotanical data from 40 aceramic Neolithic sites in southwestern Asia and southeastern Europe shows that there are vegetational signatures that characterize the different geographical regions occupied by the Early Neolithic farmers. On this basis it is argued that the compositional similarities of the crop package between the Levantine core, Cyprus, and Greece are indicative of both the routes of migration of early farming groups and the early agricultural practices of Europes first farmers.