Puncture, punctured or puncturing may refer to:
- a flat tyre in British English (US English "flat tire" or just "flat")
- a penetrating wound caused by pointy objects as nails or needles
- Lumbar puncture, also known as a spinal tap
- Puncture (band), an English punk band
- Puncture (film), a 2011 American film starring Chris Evans
- Puncture (topology), the removal of a finite set of points from a manifold
- Punctured neighbourhood, in general topology
- in coding theory, a punctured code, in which some of the bits of the data stream have been removed
- Pneumothorax, also known as punctured lung