Tupelo is a genus of trees.

Tupelo may also refer to:

• Tupelo, Mississippi
• Tupelo, Oklahoma
• "Tupelo" (Nick Cave & The Bad Seeds song)
• Tupelo honey, a monofloral varietal honey
• Tupelo Honey, an album by Van Morrison
• Tupelo Honey (band), a Canadian rock band
• Tupelo Music Hall, a concert venue in New Hampshire
• Uncle Tupelo, an alternative country band