Protein-coding gene in the species Homo sapiens
Sin3A-associated protein, 30kDa , also known as SAP30 , is a protein which in humans is encoded by the SAP30 gene .[5]
Function [ edit ] Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3A , SAP18 , HDAC1 , HDAC2 , RbAp46 , RbAp48 , and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3.[5]
Mammals have one paralog of SAP30, named SAP30-like (SAP30L ), which shares 70% sequence identity with SAP30.[6] [7]
Proteins of the SAP30 family (SAP30 proteins) have a functional nucleolar localization signal and they are able to target Sin3A to the nucleolus.[7] [8] zinc finger proteins and thus exemplifies the molecular mechanism how these lipids can contribute for gene regulation.[8] [9]
Interactions [ edit ] SAP30 has been shown to interact with:
References [ edit ]
^ a b c GRCh38: Ensembl release 89: ENSG00000164105 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000031609 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ a b "Entrez Gene: SAP30 Sin3A-associated protein, 30kDa" .^ Lindfors K, Viiri KM, Niittynen M, Heinonen TY, Mäki M, Kainulainen H (December 2003). "TGF-beta induces the expression of SAP30L, a novel nuclear protein" . BMC Genomics . 4 (1): 53. doi :10.1186/1471-2164-4-53 PMC 319701 PMID 14680513 . ^ a b Viiri KM, Korkeamäki H, Kukkonen MK, Nieminen LK, Lindfors K, Peterson P, Mäki M, Kainulainen H, Lohi O (2006). "SAP30L interacts with members of the Sin3A corepressor complex and targets Sin3A to the nucleolus" . Nucleic Acids Research . 34 (11): 3288–98. doi :10.1093/nar/gkl401 . PMC 1500868 PMID 16820529 . ^ a b Viiri KM, Jänis J, Siggers T, Heinonen TY, Valjakka J, Bulyk ML, Mäki M, Lohi O (January 2009). "DNA-binding and -bending activities of SAP30L and SAP30 are mediated by a zinc-dependent module and monophosphoinositides" . Molecular and Cellular Biology . 29 (2): 342–56. doi :10.1128/MCB.01213-08 . PMC 2612513 PMID 19015240 . ^ "Haku Tampereen yliopiston väitöskirjatietokannasta" . Archived from the original on 25 May 2011. Retrieved 24 April 2009 .^ a b Huang NE, Lin CH, Lin YS, Yu WC (June 2003). "Modulation of YY1 activity by SAP30". Biochemical and Biophysical Research Communications . 306 (1): 267–75. doi :10.1016/s0006-291x(03)00966-5 . PMID 12788099 . ^ a b c d e Zhang Y, Sun ZW, Iratni R, Erdjument-Bromage H, Tempst P, Hampsey M, Reinberg D (June 1998). "SAP30, a novel protein conserved between human and yeast, is a component of a histone deacetylase complex" . Molecular Cell . 1 (7): 1021–31. doi :10.1016/s1097-2765(00)80102-1 PMID 9651585 . ^ a b c d e Zhang Y, Ng HH, Erdjument-Bromage H, Tempst P, Bird A, Reinberg D (August 1999). "Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation" . Genes & Development . 13 (15): 1924–35. doi :10.1101/gad.13.15.1924 . PMC 316920 PMID 10444591 . ^ Swanson KA, Knoepfler PS, Huang K, Kang RS, Cowley SM, Laherty CD, Eisenman RN, Radhakrishnan I (August 2004). "HBP1 and Mad1 repressors bind the Sin3 corepressor PAH2 domain with opposite helical orientations". Nature Structural & Molecular Biology . 11 (8): 738–46. doi :10.1038/nsmb798 . PMID 15235594 . S2CID 44324333 . ^ a b Yochum GS, Ayer DE (July 2001). "Pf1, a novel PHD zinc finger protein that links the TLE corepressor to the mSin3A-histone deacetylase complex" . Molecular and Cellular Biology . 21 (13): 4110–8. doi :10.1128/MCB.21.13.4110-4118.2001 . PMC 87072 PMID 11390640 . ^ a b c d e f Kuzmichev A, Zhang Y, Erdjument-Bromage H, Tempst P, Reinberg D (February 2002). "Role of the Sin3-histone deacetylase complex in growth regulation by the candidate tumor suppressor p33(ING1)" . Molecular and Cellular Biology . 22 (3): 835–48. doi :10.1128/mcb.22.3.835-848.2002 . PMC 133546 PMID 11784859 . ^ a b Laherty CD, Billin AN, Lavinsky RM, Yochum GS, Bush AC, Sun JM, Mullen TM, Davie JR, Rose DW, Glass CK, Rosenfeld MG, Ayer DE, Eisenman RN (July 1998). "SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors" . Molecular Cell . 2 (1): 33–42. doi :10.1016/s1097-2765(00)80111-2 PMID 9702189 . ^ Underhill C, Qutob MS, Yee SP, Torchia J (December 2000). "A novel nuclear receptor corepressor complex, N-CoR, contains components of the mammalian SWI/SNF complex and the corepressor KAP-1" . The Journal of Biological Chemistry . 275 (51): 40463–70. doi :10.1074/jbc.M007864200 PMID 11013263 . ^ Fleischer TC, Yun UJ, Ayer DE (May 2003). "Identification and characterization of three new components of the mSin3A corepressor complex" . Molecular and Cellular Biology . 23 (10): 3456–67. doi :10.1128/mcb.23.10.3456-3467.2003 . PMC 164750 PMID 12724404 .
Further reading [ edit ]
Ayer DE, Lawrence QA, Eisenman RN (March 1995). "Mad-Max transcriptional repression is mediated by ternary complex formation with mammalian homologs of yeast repressor Sin3" . Cell . 80 (5): 767–76. doi :10.1016/0092-8674(95)90355-0 PMID 7889570 . S2CID 8749951 . Zhang Y, Iratni R, Erdjument-Bromage H, Tempst P, Reinberg D (May 1997). "Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex" . Cell . 89 (3): 357–64. doi :10.1016/S0092-8674(00)80216-0 PMID 9150135 . Zhang Y, Sun ZW, Iratni R, Erdjument-Bromage H, Tempst P, Hampsey M, Reinberg D (June 1998). "SAP30, a novel protein conserved between human and yeast, is a component of a histone deacetylase complex" . Molecular Cell . 1 (7): 1021–31. doi :10.1016/S1097-2765(00)80102-1 PMID 9651585 . Laherty CD, Billin AN, Lavinsky RM, Yochum GS, Bush AC, Sun JM, Mullen TM, Davie JR, Rose DW, Glass CK, Rosenfeld MG, Ayer DE, Eisenman RN (July 1998). "SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors" . Molecular Cell . 2 (1): 33–42. doi :10.1016/S1097-2765(00)80111-2 PMID 9702189 . Zhang Y, LeRoy G, Seelig HP, Lane WS, Reinberg D (October 1998). "The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities" . Cell . 95 (2): 279–89. doi :10.1016/S0092-8674(00)81758-4 PMID 9790534 . S2CID 18786866 . Tong JK, Hassig CA, Schnitzler GR, Kingston RE, Schreiber SL (October 1998). "Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex". Nature . 395 (6705): 917–21. Bibcode :1998Natur.395..917T . doi :10.1038/27699 . PMID 9804427 . S2CID 4355885 . Hsieh JJ, Zhou S, Chen L, Young DB, Hayward SD (January 1999). "CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex" . Proceedings of the National Academy of Sciences of the United States of America . 96 (1): 23–8. Bibcode :1999PNAS...96...23H . doi :10.1073/pnas.96.1.23 PMC 15086 PMID 9874765 . Boutell JM, Thomas P, Neal JW, Weston VJ, Duce J, Harper PS, Jones AL (September 1999). "Aberrant interactions of transcriptional repressor proteins with the Huntington's disease gene product, huntingtin" . Human Molecular Genetics . 8 (9): 1647–55. doi :10.1093/hmg/8.9.1647 PMID 10441327 . Zhang Y, Ng HH, Erdjument-Bromage H, Tempst P, Bird A, Reinberg D (August 1999). "Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation" . Genes & Development . 13 (15): 1924–35. doi :10.1101/gad.13.15.1924 . PMC 316920 PMID 10444591 . Krithivas A, Young DB, Liao G, Greene D, Hayward SD (October 2000). "Human herpesvirus 8 LANA interacts with proteins of the mSin3 corepressor complex and negatively regulates Epstein-Barr virus gene expression in dually infected PEL cells" . Journal of Virology . 74 (20): 9637–45. doi :10.1128/JVI.74.20.9637-9645.2000 . PMC 112396 PMID 11000236 . Underhill C, Qutob MS, Yee SP, Torchia J (December 2000). "A novel nuclear receptor corepressor complex, N-CoR, contains components of the mammalian SWI/SNF complex and the corepressor KAP-1" . The Journal of Biological Chemistry . 275 (51): 40463–70. doi :10.1074/jbc.M007864200 PMID 11013263 . Humphrey GW, Wang Y, Russanova VR, Hirai T, Qin J, Nakatani Y, Howard BH (March 2001). "Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1" . The Journal of Biological Chemistry . 276 (9): 6817–24. doi :10.1074/jbc.M007372200 PMID 11102443 . Skowyra D, Zeremski M, Neznanov N, Li M, Choi Y, Uesugi M, Hauser CA, Gu W, Gudkov AV, Qin J (March 2001). "Differential association of products of alternative transcripts of the candidate tumor suppressor ING1 with the mSin3/HDAC1 transcriptional corepressor complex" . The Journal of Biological Chemistry . 276 (12): 8734–9. doi :10.1074/jbc.M007664200 PMID 11118440 . Yochum GS, Ayer DE (July 2001). "Pf1, a novel PHD zinc finger protein that links the TLE corepressor to the mSin3A-histone deacetylase complex" . Molecular and Cellular Biology . 21 (13): 4110–8. doi :10.1128/MCB.21.13.4110-4118.2001 . PMC 87072 PMID 11390640 . Kuzmichev A, Zhang Y, Erdjument-Bromage H, Tempst P, Reinberg D (February 2002). "Role of the Sin3-histone deacetylase complex in growth regulation by the candidate tumor suppressor p33(ING1)" . Molecular and Cellular Biology . 22 (3): 835–48. doi :10.1128/MCB.22.3.835-848.2002 . PMC 133546 PMID 11784859 . Saito M, Ishikawa F (September 2002). "The mCpG-binding domain of human MBD3 does not bind to mCpG but interacts with NuRD/Mi2 components HDAC1 and MTA2" . The Journal of Biological Chemistry . 277 (38): 35434–9. doi :10.1074/jbc.M203455200 PMID 12124384 . Wysocka J, Myers MP, Laherty CD, Eisenman RN, Herr W (April 2003). "Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1" . Genes & Development . 17 (7): 896–911. doi :10.1101/gad.252103 . PMC 196026 PMID 12670868 . Huang NE, Lin CH, Lin YS, Yu WC (June 2003). "Modulation of YY1 activity by SAP30". Biochemical and Biophysical Research Communications . 306 (1): 267–75. doi :10.1016/S0006-291X(03)00966-5 . PMID 12788099 . Sironi E, Cerri A, Tomasini D, Sirchia SM, Porta G, Rossella F, Grati FR, Simoni G (April 2004). "Loss of heterozygosity on chromosome 4q32-35 in sporadic basal cell carcinomas: evidence for the involvement of p33ING2/ING1L and SAP30 genes". Journal of Cutaneous Pathology . 31 (4): 318–22. doi :10.1111/j.0303-6987.2004.0187.x . PMID 15005689 . S2CID 2520678 .
