Staehelin or Stähelin is a surname. Notable people with the surname include:

• Adolf Staehelin (1901–1965), Swiss chess player
• Jenö Staehelin (born 1940), Swiss diplomat and lawyer
• Lucas Andrew Staehelin (born 1939), Australian-born Swiss American cell biologist
• Martin Staehelin (born 1937), Swiss musicologist

• August Stähelin (1812–1886), Swiss politician
• Felix Stähelin (1873–1952), Swiss historian
• Hartmann F. Stähelin (1925–2011), Swiss pharmacologist
• Helene Stähelin (1891–1970), Swiss mathematician
• Johann Jakob Stähelin (1797–1875), Swiss theologian
• Rudolf Stähelin (1875–1943), Swiss physician