Mammalian protein found in Homo sapiens
Bifunctional purine biosynthesis protein PURH is a protein that in humans is encoded by the ATIC gene .[5] [6] [7]
ATIC encodes an enzyme which generates inosine monophosphate from aminoimidazole carboxamide ribonucleotide .
It has two functions:
EC 2.1.2.3 - 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
EC 3.5.4.10 - IMP cyclohydrolase
References [ edit ]
^ a b c GRCh38: Ensembl release 89: ENSG00000138363 – Ensembl , May 2017
^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026192 – Ensembl , May 2017
^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ Rayl EA, Moroson BA, Beardsley GP (Mar 1996). "The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping" . J Biol Chem . 271 (4): 2225–33. doi :10.1074/jbc.271.4.2225 . PMID 8567683 .
^ Sugita T, Aya H, Ueno M, Ishizuka T, Kawashima K (Nov 1997). "Characterization of molecularly cloned human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase". J Biochem . 122 (2): 309–13. doi :10.1093/oxfordjournals.jbchem.a021754 . PMID 9378707 .
^ "Entrez Gene: ATIC 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase" .
Further reading [ edit ]
Rasmussen HH, van Damme J, Puype M, Gesser B, Celis JE, Vandekerckhove J (1993). "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes". Electrophoresis . 13 (12): 960–9. doi :10.1002/elps.11501301199 . PMID 1286667 . S2CID 41855774 .
Yamauchi M, Seki N, Mita K, Saito T, Tsuji S, Hongo E, Morimyo M, Shiomi T, Koyama H (1996). "Isolation of human purH gene expressed in the rodent transformant cells by subtractive enrichment of 3'-untranslated region of human transcript" . DNA Res . 2 (6): 269–75. doi :10.1093/dnares/2.6.269 . PMID 8867801 .
Beardsley GP, Rayl EA, Gunn K, Moroson BA, Seow H, Anderson KS, Vergis J, Fleming K, Worland S (1998). "Structure and Functional Relationships in Human pur H". Purine and Pyrimidine Metabolism in Man IX . Advances in Experimental Medicine and Biology. Vol. 431. pp. 221–6. doi :10.1007/978-1-4615-5381-6_43 . ISBN 978-0-306-45778-4 . PMID 9598063 .
Vergis JM, Bulock KG, Fleming KG, Beardsley GP (2001). "Human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase. A bifunctional protein requiring dimerization for transformylase activity but not for cyclohydrolase activity" . J. Biol. Chem . 276 (11): 7727–33. doi :10.1074/jbc.M009940200 . PMID 11096114 .
Bulock KG, Beardsley GP, Anderson KS (2002). "The kinetic mechanism of the human bifunctional enzyme ATIC (5-amino-4-imidazolecarboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase). A surprising lack of substrate channeling" . J. Biol. Chem . 277 (25): 22168–74. doi :10.1074/jbc.M111964200 . PMID 11948179 .
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 . PMC 139241 . PMID 12477932 .
Cheong CG, Wolan DW, Greasley SE, Horton PA, Beardsley GP, Wilson IA (2004). "Crystal structures of human bifunctional enzyme aminoimidazole-4-carboxamide ribonucleotide transformylase/IMP cyclohydrolase in complex with potent sulfonyl-containing antifolates" . J. Biol. Chem . 279 (17): 18034–45. doi :10.1074/jbc.M313691200 . PMID 14966129 .
Marie S, Heron B, Bitoun P, Timmerman T, Van Den Berghe G, Vincent MF (2004). "AICA-Ribosiduria: A Novel, Neurologically Devastating Inborn Error of Purine Biosynthesis Caused by Mutation of ATIC" . Am. J. Hum. Genet . 74 (6): 1276–81. doi :10.1086/421475 . PMC 1182092 . PMID 15114530 .
Dervieux T, Furst D, Lein DO, Capps R, Smith K, Walsh M, Kremer J (2004). "Polyglutamation of methotrexate with common polymorphisms in reduced folate carrier, aminoimidazole carboxamide ribonucleotide transformylase, and thymidylate synthase are associated with methotrexate effects in rheumatoid arthritis" . Arthritis Rheum . 50 (9): 2766–74. doi :10.1002/art.20460 . PMID 15457444 .
Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 . PMID 15489334 .
Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry" . Mol. Syst. Biol . 3 (1): 89. doi :10.1038/msb4100134 . PMC 1847948 . PMID 17353931 .
External links [ edit ]
PDB gallery
1p4r : Crystal Structure of Human ATIC in complex with folate-based inhibitor BW1540U88UD
1pkx : Crystal Structure of human ATIC in complex with XMP
1pl0 : Crystal structure of human ATIC in complex with folate-based inhibitor, BW2315U89UC