Thulasi or Tulasi may refer to:
- Tulasi or Ocimum tenuiflorum, an aromatic plant in the family Lamiaceae
- Tulasi (film), a 2007 Tollywood film by Boyapati Srinu
- Thulasi (1987 film), a 1987 Tamil film starring Murali and Seetha
- Thulasi (1976 film), a 1976 Indian Kannada film
- Tulsidas or Tulasidas (1532–1623), Indian saint and poet
- Thulasidas, Indian film director
- Tulasi (actress) (born 1967), Indian film actress