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Not to be confused with Curcumin.
Curculin-1
A curculin homodimer. From PDB: 2DPF​.
Identifiers
OrganismMolineria latifolia
SymbolCURC_CURLA
PDB2DPF
UniProtP19667
Search for
StructuresSwiss-model
DomainsInterPro
Curculin-2
Identifiers
OrganismMolineria latifolia
SymbolCURC2_CURLA
PDB2D04
UniProtQ6F495
Search for
StructuresSwiss-model
DomainsInterPro

Curculin or neoculin is a sweet protein that was discovered and isolated in 1990 from the fruit of Curculigo latifolia (Hypoxidaceae),[1] a plant from Malaysia. Like miraculin, curculin exhibits taste-modifying activity; however, unlike miraculin, it also exhibits a sweet taste by itself. After consumption of curculin, water and sour solutions taste sweet. The plant is referred to locally as 'Lumbah' or 'Lemba'.

Protein structure[edit]

The active form of curculin is a heterodimer consisting of two monomeric units connected through two disulfide bridges. The mature monomers each consist of a sequence of 114 amino acids, weighing 12.5 kDa (curculin 1) and 12.7 kDa (curculin 2), respectively. While each of the two isoforms is capable of forming a homodimer, these do not possess the sweet taste nor the taste-modifying activity of the heterodimeric form.[2] To avoid confusion, the heterodimeric form is sometimes referred to as "neoculin".

  • 1, 1-50: DNVLLSGQTL HADHSLQAGA YTLTIQNKCN LVKYQNGRQI WASNTDRRGS
  • 1, 51-100: GCRLTLLSDG NLVIYDHNNN DVWGSACWGD NGKYALVLQK DGRFVIYGPV
  • 1, 101-114: LWSLGPNGCR RVNG
  • 2, 1-50: DSVLLSGQTL YAGHSLTSGS YTLTIQNNCN LVKYQHGRQI WASDTDGQGS
  • 2, 51-100: QCRLTLRSDG NLIIYDDNNM VVWGSDCWGN NGTYALVLQQ DGLFVIYGPV
  • 2, 101-113: LWPLGLNGCR SLN

Amino acid sequence of sweet proteins curculin-1 and curculin-2 adapted from Swiss-Prot biological database of protein sequences. Intra-chain disulfide bonds in bold, inter-chain disulfide bonds underlined.[3]

Sweetness properties[edit]

Curculin is considered to be a high-intensity sweetener, with a reported relative sweetness of 430-2070 times sweeter than sucrose on a weight basis.[1][4][5]

A sweet taste, equivalent to a 6.8% or 12% sucrose solution, was observed after holding curculin in the mouth in combination with clear water or acidified water (citric acid), respectively. The sweet taste lasts for 5 minutes with water and 10 minutes with an acidic solution.[1]

The taste-modifying activity of curculin is reduced in the presence of ions with two positive charges (such as Ca2+ and Mg2+) in neutral pH solutions, although these ions have no effect in acidic solutions. In the same way, monovalent ions (such as Na+ and Cl−) have no effect in solutions with either neutral or acidic pH.[1][5]

Although the "sweet-inducing" mechanism is unknown, it is believed that one active site of curculin strongly binds to the taste receptor membranes while a second active site fits into the sweet receptor site. The latter site is thought to be responsible for the induction of sweetness. Presence of Ca2+ and/or Mg2+, water and acids tune the binding of the active site of curculin to the receptor site and therefore modify perceived sweetness.[5] Curculin appears to use a unique binding site at the amino terminal of TAS1R3.[6]

As a sweetener[edit]

Like most proteins, curculin is susceptible to heat. At a temperature of 50 °C (122 °F) the protein starts to degrade and lose its "sweet-tasting" and "taste-modifying" properties, so it is not a good candidate for use in hot or processed foods. However, below this temperature both properties of curculin are unaffected in basic and acidic solutions,[5] so it has potential for use in fresh foods and as a table-top sweetener.

Because curculin is not widely found in nature, efforts are underway to produce a recombinant form of the protein. In 1997, curculin was expressed in E. coli and yeast, but the recombinant protein did not exhibit "sweet-tasting" or "taste-modifying" activity.[7] However, a 2004 study obtained a recombinant curculin, expressed in E. coli, exhibiting "taste-modifying" and "sweet-tasting" properties.[2]

In addition to challenges related to commercial production of the protein, there are many regulatory and legal issues remaining to be resolved before it can be marketed as a sweetener. Curculin currently has no legal status in European Union and United States. However it is approved in Japan as a harmless additive, according to the List of Existing Food Additives established by the Ministry of Health and Welfare (English publication by JETRO).

See also[edit]

References[edit]

  1. ^ a b c d Yamashita H, Theerasilp S, Aiuchi T, et al. (September 1990). "Purification and complete amino acid sequence of a new type of sweet protein taste-modifying activity, curculin". The Journal of Biological Chemistry. 265 (26): 15770–5. doi:10.1016/S0021-9258(18)55464-8. PMID 2394746.
  2. ^ a b Suzuki M, Kurimoto E, Nirasawa S, et al. (August 2004). "Recombinant curculin heterodimer exhibits taste-modifying and sweet-tasting activities". FEBS Letters. 573 (1–3): 135–8. doi:10.1016/j.febslet.2004.07.073. PMID 15327988.
  3. ^ Universal protein resource accession number P19667 for "Curculin-1" at UniProt. Universal protein resource accession number Q6F495 for "Curculin-2" at UniProt.
  4. ^ Kurihara Y (1992). "Characteristics of antisweet substances, sweet proteins, and sweetness-inducing proteins". Critical Reviews in Food Science and Nutrition. 32 (3): 231–52. doi:10.1080/10408399209527598. PMID 1418601.
  5. ^ a b c d Yamashita H, Akabane T, Kurihara Y (April 1995). "Activity and stability of a new sweet protein with taste-modifying action, curculin". Chemical Senses. 20 (2): 239–43. doi:10.1093/chemse/20.2.239. PMID 7583017.
  6. ^ Koizumi A, Nakajima K, Asakura T, et al. (29 June 2007). "Taste-modifying sweet protein, neoculin, is received at human T1R3 amino terminal domain". Biochemical and Biophysical Research Communications. 358 (2): 585–9. doi:10.1016/j.bbrc.2007.04.171. PMID 17499612.
  7. ^ Kurihara Y, Nirasawa S (1997). "Structures and activities of sweetness-inducing substances (miraculin, curculin, strogin) and the heat-stable sweet protein, mabinlin". Foods and Food Ingredients Journal of Japan: 67–74.

External links[edit]

  • Media related to Curculin at Wikimedia Commons
source: https://en.wikipedia.org/wiki/Curculin

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