Cannabis Sativa

thcscience_admin
Brazzein
Solution NMR structure of the brazzein protein.[1]
Identifiers
OrganismPentadiplandra brazzeana
SymbolMONA_DIOCU
PDB1BRZ
UniProtP56552
Search for
StructuresSwiss-model
DomainsInterPro

Brazzein is a protein found in the West African fruit of Oubli (Pentadiplandra brazzeana Baillon). It was first isolated by the University of Wisconsin–Madison in 1994.[2]

Brazzein is found in the extracellular region, in the pulp tissue surrounding the seeds. With pentadin, discovered in 1989, brazzein is the second sweet-tasting protein discovered in the Oubli fruit.[3]

Like the other sweet proteins discovered in plants, such as monellin and thaumatin, it is extremely sweet compared to commonly used sweeteners (500 to 2000 times sweeter than sucrose).[4] The fruit tastes sweet to humans, monkeys, and bonobos, but gorillas have mutations in their sweetness receptors so that they do not find brazzein sweet, and they are not known to eat the fruit.[5][6]

Traditional use[edit]

The Oubli plant (from which the protein was isolated) grows in Gabon and Cameroon, where its fruit has been consumed by the apes and local people for a long time. Due to brazzein and pentadin, the berries of the plant are incredibly sweet. Locals call them "Oubli" (French for "forgot") in their vernacular language because their taste is said to encourage nursing infants to forget their mother's milk,[7] as once they eat them they are said to forget to come back to the village to see their mother.[8]

Protein structure[edit]

The monomer protein, consisting of 54 amino acid residues, is the smallest of the sweet proteins with a molecular weight of 6.5 kDa.[2] The amino acid sequence of brazzein, adapted from the Swiss-Prot biological database of protein, is as follows: QDKCKKVYEN YPVSKCQLAN QCNYDCKLDK HARSGECFYD EKRNLQCICD YCEY[9]

The structure of brazzein was determined by proton nuclear magnetic resonance (NMR) at a pH of 5.2 and 22 °C. Brazzein has four evenly spaced disulfide bonds and no sulfhydryl groups.

3D analysis of brazzein showed one alpha-helix and three strands of anti-parallel beta sheet. This is not superficially similar to either of the other two sweet-tasting proteins, monellin and thaumatin.[10]

However, a recent 3D study shows that these three proteins possess similar "sweet fingers" believed to elicit the sweet taste.[11]

Residues 29–33 and 39–43, plus residue 36, as well as the C-terminus were found to be involved in the sweet taste of the protein. The charge of the protein also plays an important role in its interaction with the sweet taste receptor.[2]

Based on this knowledge a synthesised improved brazzein, called pGlu-1-brazzein, was reported to be twice as sweet as the natural counterpart.[12]

Sweetness properties[edit]

On a weight basis, brazzein is 500 to 2000 times sweeter than sucrose, compared to 10% sucrose and 2% sucrose solution respectively.[10]

Its sweet perception is more similar to sucrose than that of thaumatin, with a clean sweet taste, lingering aftertaste, and slight delay (longer than aspartame) in an equi-sweet solution.[13]

Brazzein is stable over a broad pH range from 2.5 to 8[14] and heat stable at 98°C for 2 hours.[2]

As a sweetener[edit]

Brazzein represents an alternative to available low-calorie sweeteners. As a protein, it is safe for diabetics. It is also very soluble in water (>50 mg/mL).[14]

When blended with other sweeteners, such as aspartame and stevia, brazzein reduces side aftertaste and complements their flavor.[15]

Its taste profile is closer to sucrose than other natural sweeteners (apart from thaumatin). Unlike other sweet-tasting proteins, it can withstand heat, making it more suitable for industrial food processing.[16]

Papers have been published showing it can be made in a laboratory using peptide synthesis[10] and recombinant proteins were successfully produced via E. coli.[17]

The Texas companies Prodigene and Nectar Worldwide were among the licensees to use Wisconsin Alumni Research Foundation patents on brazzein, and genetically engineer it into maize. Brazzein then can be commercially extracted from the maize through ordinary milling. Approximately one ton of maize yields 1-2 kilograms of brazzein. It can also be engineered into plants like wheat to make pre-sweetened grains, e.g. for cereals.[16]

A company was formed to bring it to market as a sweetener in 2008, which initially said it would start selling the product by 2010 once it obtained agreement from the FDA that its brazzein was generally recognized as safe (GRAS).[18]

In 2024, the brand Oobli received the first GRAS certification from the FDA, with no potential concerns for consumption being raised. [19]

See also[edit]

References[edit]

  1. ^ PDB: 2brz​; Caldwell JE, Abildgaard F, Dzakula Z, Ming D, Hellekant G, Markley JL (June 1998). "Solution structure of the thermostable sweet-tasting protein brazzein". Nat. Struct. Biol. 5 (6): 427–31. doi:10.1038/nsb0698-427. PMID 9628478. S2CID 385103.
  2. ^ a b c d Ming D, Hellekant G (November 1994). "Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B". FEBS Lett. 355 (1): 106–8. doi:10.1016/0014-5793(94)01184-2. PMID 7957951. S2CID 6650703.
  3. ^ van der Wel H, Larson G, Hladik A, Hladik CM, Hellekant G, Glaser D (1989). "Isolation and characterization of pentadin, the sweet principle of Pentadiplandra brazzeana Baillon" (PDF). Chem. Senses. 14 (1): 75–79. doi:10.1093/chemse/14.1.75.
  4. ^ Faus I, Sisniega H (2004). "Sweet-tasting Proteins" (PDF). In Hofrichter M, Steinbüchel A (eds.). Biopolymers: Polyamides and Complex Proteinaceous Materials II (8th ed.). Weinheim: Wiley-VCH. pp. 203–209. ISBN 978-3-527-30223-9. Archived from the original (PDF) on 2009-09-20. Retrieved 2007-09-28.
  5. ^ Guevara EE, Veilleux CC, Saltonstall K, Caccone A, Mundy NI, Bradley BJ (Jul 2016). "Potential arms race in the coevolution of primates and angiosperms: brazzein sweet proteins and gorilla taste receptors". American Journal of Physical Anthropology. 161 (1): 181–185. doi:10.1002/ajpa.23046. PMID 27393125.
  6. ^ "Gorillas may have evolved a way to beat a cheating berry plant". New Scientist. Jul 23, 2016.
  7. ^ Stein J (2002-11-04). "UW–Madison professor makes a sweet discovery". Wisconsin State Journal.[permanent dead link]
  8. ^ Hladik CM, Hladik A (1988). "Sucres et "faux sucres" de la forêt équatoriale : évolution et perception des produits sucrés par les populations forestières d'Afrique". Journal d'Agriculture Tropicale et de Botanique Appliquée (FRA). 35: 51–66. doi:10.3406/jatba.1988.6676. S2CID 163717669. Archived from the original on 2011-07-26. Retrieved 2007-10-01.
  9. ^ UniProtKB/Swiss-Prot database entry #PP56552
  10. ^ a b c Izawa H, Ota M, Kohmura M, Ariyoshi Y (July 1996). "Synthesis and characterization of the sweet protein brazzein". Biopolymers. 39 (1): 95–101. doi:10.1002/(SICI)1097-0282(199607)39:1<95::AID-BIP10>3.0.CO;2-B. PMID 8924630.
  11. ^ Tancredi T, Pastore A, Salvadori S, Esposito V, Temussi PA (June 2004). "Interaction of sweet proteins with their receptor. A conformational study of peptides corresponding to loops of brazzein, monellin and thaumatin". Eur. J. Biochem. 271 (11): 2231–40. doi:10.1111/j.1432-1033.2004.04154.x. PMID 15153113.
  12. ^ Assadi-Porter FM, Aceti DJ, Markley JL (April 2000). "Sweetness determinant sites of brazzein, a small, heat-stable, sweet-tasting protein". Arch. Biochem. Biophys. 376 (2): 259–65. doi:10.1006/abbi.2000.1726. PMID 10775411.
  13. ^ Pfeiffer JF, Boulton RB, Noble AC (2000). "Modelling the sweetness response using time-intensity data". Food Quality and Preference. 11 (1): 129–138. doi:10.1016/S0950-3293(99)00036-1.
  14. ^ a b Birch, Gordon Gerard (2000). Ingredients Handbook - Sweeteners (Ingredients Handbook Series). Leatherhead Food Research Association. ISBN 978-0-905748-90-0.
  15. ^ Hellekant G, Danilova V (2005). "Brazzein a Small, Sweet Protein: Discovery and Physiological Overview". Chem. Senses. 30 (Supplement 1): i88–i89. doi:10.1093/chemse/bjh127. PMID 15738210.
  16. ^ a b US patent 5326580, Hellekant BG, Ming D, "Brazzein sweetener", issued 1994-07-05 
  17. ^ Assadi-Porter FM, Aceti DJ, Cheng H, Markley JL (April 2000). "Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant origin". Arch. Biochem. Biophys. 376 (2): 252–8. doi:10.1006/abbi.2000.1725. PMID 10775410.
  18. ^ Hills, Sarah (24 June 2008). "New sweetener to hit market hungry for alternatives". FoodNavigator-USA.com.
  19. ^ "Oobli Becomes Receives FDA "No Questions" Letter for Novel Sweet Protein Produced via Precision Fermentation | Prepared Foods". www.preparedfoods.com. Retrieved 2024-03-29.

External links[edit]

  • Media related to Brazzein at Wikimedia Commons
source: https://en.wikipedia.org/wiki/Brazzein

Leave a Reply